In addition it can also solubilise k, zn, fe and mn and is also found to have antagonistic activity against select. Isolation and application of salttolerant or halophilic psb will facilitate the development of salinealkali soil based agriculture. Phosphate solubilizing microbes have been reported for plant growth promoting and enhancing yield kapoor, mishra, and kuhkreja 1989. Inorganicphosphatesolubilizing bacteria ipsb have been isolated from the rhizosphere of many terrestrial plants sundararao and sinha 1963. Isolation and solubilisation of inorganic phosphate by. Phosphorus is an essential element for plant development and growth making up about 0. Phosphate were applied to soil compared with all other treatments. Release of p by psb from insoluble and fixed adsorbed forms is an import aspect regarding p availability in soils. A considerable number of bacterial species are able to exert a beneficial effect upon plant growth. Characteristics of phosphate solubilization by phosphate.
Soil inoculation with phosphate solubilizing bacteria. Mostly they are associated with the plant rhizosphere, so they are called as rhizobacteria. Phosphorus p is one of the major bioelements limiting agricultural production. Pdf isolation and screening of phosphate solubilizing. In soil, phosphorus p is sequestered by adsorption to the surface of soil particles and. However as the ph of the soil environment increases the divalent and trivalent forms of pi hpo 42 and hpo 43 respectively occur. These phosphate solubilizing bacteria psb are being used as biofertilizer since 1950s. Assessment of their effect on growth promotion and phosphorous uptake of mung bean vigna radiata l. The aims of this study was to isolate psb from oil palm rhizosphere and to conduct a comparative analysis of the solubility of inorganic phosphates. After 47 likely in legume roots in which nodulation takes place. Given the negative environmental impacts of chemical fertilizers and increasing costs, the utilization of phosphatesolubilizing bacteria psb is advantageous. The result showed that the microbial population ranged. Phosphate solubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Isolation, screening and characterization of phosphate.
The present study was aimed at isolating and characterizing phosphate solubilizing fungi from different rhizospheres using both solid and liquid pikovskaya pvk medium. A study was undertaken to investigate the occurrence of phosphate solubilizing bacteria psb and nitrogenfixing bacteria nfb from soil samples of wamena biological garden wbig. For isolation of phosphate solubilizing microbes, 1g rhizosphere soil was suspended in 100ml of distilled water. Phosphate solubilizing bacteria from sunflower rhizosphere 439 centrifuged at 10,000 rpm for 15 minutes. Phosphate solubilising bacteria psb are useful for plant growth. Phosphate solubilization characteristics of efficient. Isolation and enumeration of populations of phosphatesolubilizing bacteria from the rhizosphere and nonrhizosphere soil samples of foxtail millet. Phosphate solubilizing bacteria from subtropical soil and. Phosphate solubilizing microbes are found in soil mostisolation of phosphate solubilizing bacteria. Phosphate solubilizing bacteria an overview sciencedirect. The collected samples were carefully packed in paper bags and stored at room temperature. Organic phosphate solubilization is also called mineralization of organic phosphorus, and it occurs in soil at the expense of plant and animal remains, which contain a large. Coochbehar, jalpaiguri and alipurduar from different crop rhizosphere. How to isolate phosphate solubilizing bacteria youtube.
The present study focuses on the phosphatesolubilizing capacity of bacteria and fungi in rhizospheres soil samples obtained from. The integrated use of rock phosphate and psm add 2030 kg p2o5ha. The present study focuses on the phosphate solubilizing capacity of bacteria and fungi in rhizospheres soil samples obtained from. From our study, we conclude that the phosphatesolubilizing gramnegative bacteria were present in maize, corn, jasmine, and onion fields and enrich soil soluble phosphate. More studies are warranted to identify and understand the significance and mechanism underlying the formation of soluble phosphate by psb and its benefits as bioinoculants. The inorganic and organic phosphate solubilization potentials of twenty competent phosphate solubilizing azotobacter isolates were assessed. Performance of phosphatesolubilizing bacteria in soil. The monovalent anion phosphate h 2 po 4is a major soluble form of inorganic phosphate, which usually occurs at lower ph. Phosphate solubilizing bacteria psb could be very useful for increasing mining efficiency over time. Phosphate solubilization potential of rhizosphere fungi. Characteristics of phosphate solubilization by phosphatesolubilizing bacteria isolated from agricultural chili soil and their efficiency on the growth of chili capsicum frutescens l.
Phosphate solubilizing bacteria psb are beneficial bacteria capable of solubilizing inorganic phosphorus from insoluble compounds. Phosphatesolubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. The serially diluted soil samples were plated on standard agar medium ph 6. Thus, the synthesis and discharge of organic acid by the psm strains into the surrounding environment acidify the cells and their. Hua rua worapon surapat a, charida pukahuta a, pongsak rattanachaikunsopon a. There are strong evidences that soil bacteria are capable of transforming soil p to the forms available to plant. Ten strains that had higher solubilization potential were selected, and they also produced indole3. The mineral nutrition of plants mainly depends on soil p content that can be assimilated as a soluble phosphate.
Phosphorus is a major essential macro element required for plants to growth and development. Of 35 phosphate solubilizing rhizobacteria, 14 isolates were selected for their solubilization diameters 0. Silicate bacteria were found to resolve potassium, silicon and aluminum from insoluble minerals alexander. Immobilization of pb by phosphatesolubilizing bacteria. This study aimed to isolate a highefficient level of phosphatesolubilizing fungus from rhizosphere soil samples phosphate mines liuyang county, hunan province, china and apply it in solubilization of rps. Collection of soil samples the field sites for collecting soil sample to isolate psm were selected randomly based. Very little of this potassium source is available for plant use. A number of heterotrophic microorganisms excreting organic acids which solubalized p that chelate cationic partners of p ions and release the p directly into solution he et al.
Phosphate solubilizing bacteria and their role in plant. Experimental studies found that the strain pssg6 was effective in phosphate solubilization with phosphate solubilization efficiency index e 375 8. They convert the nonavailable inorganic phosphate to its soluble form, thus increasing the availability of phosphorus by secreting organic acids like citric, oxalic, succinic. Isolation and characterization of a phosphatesolubilizing. Colonies showing phosphate solubilizing zone around them were considered as psm. Phosphate solubilizing bacteria psb play an important role in biogeochemical phosphorus cycling in both terrestrial and aquatic environments. It is important to explore a highefficient phosphate solubilizing approach with a kind of microorganisms. A way to bring phosphate saturated soils back to an environmentally safe p level is by p mining through plants.
Isolation and characterization of ralstonia pickettii a. What are examples of phosphorous solublising bacteria. Therefore, p is often regarded a limiting nutrient in agricultural soils guinazu et al. Soil microorganisms influence the availability of soil minerals, playing a central role in ion cycling and soil fertility bin lian et al.
Phosphate solubilizing bacteria psb have the potential to enhance phosphateinduced immobilization of metals to remediate contaminated soil. In a previous investigation in china, 56 bacteria species were found in root tissue, rhizoplane, and rhizosphere of moso bamboo3. The analysis of phosphate solubilizing bacteria from soil sample was carried o ut in 22 o 32 17. Moroccan journal of biology 20n 10 isolation and identification of potential phosphate solubilizing bacteria from the rhizosphere of lupinus hirsutus l. With the aim to develop biofertilizer, a total of 283 bacteria belonging to pseudomonas were isolated from the rice rhizosphere of which 67. Microorganisms play an important role in the availability of soil phosphate to the root system and enhance the mobilization of phosphate in soil richardson, 2001. After 5 days, serial dilution of the enriched medium was carried out and.
Isolation and characterization of phosphatesolubilizing. An aliquot 100l from decimal dilutions was inoculated on picovskayas medium by pour plate technique and incubated at 30oc. The bioavailability of soil inorganic phosphorus in the rhizosphere varies considerably with plant species, nutritional status of soil and ambient soil. They inhabit different soil ecosystems such as colonizing the root environment. A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china, which also. Isolation, characterization and identification of potassium solubilizing bacteria from rhizosphere soil of maize zea mays. Wilczek buddhi charana walpola 1, and minho yoon1 the aim of this work was to isolate phosphate solubilizing bacteria psb and assess their effect on the growth of mung bean. The phosphate solubilizing bacteria psb solubilizes the inorganic soil phosphates of ca, fe, and al via production of siderophores, several acids organic, and hydroxyl and carboxyl groups, and chelating them to the bound phosphates and the available calcium sharma et al. The search for life in space documentary where is the centre of the universe touch your heart 470 watching live now. It precipitates in soil as orthophosphate or is absorbed by fe and al oxides through legend exchange. Mineral phosphate solubilization several reports have examined the ability of different bacterial species to solubilize insoluble inorganic phosphate compounds, such as tricalcium phosphate, dicalcium phosphate, hydroxyapatite, and rock phosphate 38.
Pdf isolation of phosphate solubilizing microorganism. The different strains of phosphate solubilizing bacteria proteus vulgaris, k. A way to bring phosphatesaturated soils back to an environmentally safe p level is by p mining through plants. Isolation of phosphate solubilizing bacteria and fungi from. Hook and halodule pinifolia miki hartog in the vellar estuary. Although the authors did not identify phosphatesolubilizing bacteria, there is still a possibility to discover this type of bacteria in bamboo root. Plants acquire phosphorus from soil solution as phosphate anion. Homogenization of soil was carried out by keeping it on shaker for 30 minutes at 120 rpm at 27oc temperature. It is the least mobile element in plant and soil contrary to other macronutrients. Isolation and identification of phosphate solubilizing and. Phosphate solubilizing potentiality of the microorganisms capable of utilizing aluminium phosphate as a sole phosphate source. Isolation and phenotypic characterization of phosphate.
Phosphatesolubilizing bacteria psb could be very useful for increasing mining efficiency over time. Several scientists have reported the ability of different bacterial species to solubilize. Request pdf mechanisms of phosphate solubilization by psb phosphate solubilizing bacteria in soil among the major nutrients, phosphorus is by far the least mobile and available to plants in. Isolation and characterization of phosphate solubilizing.
Phosphate solubilizing bacteria psb can convert insoluble forms of phosphorus p to accessible forms. Nineteen soil samples were collected from different terai agroecological region of west bengal i. However, there is a limit on the amount of phosphate which can be added to the environment due to the issue of eutrophication. The interactive effect of phosphate solubilizing bacteria and arbuscular mycorrhizal fungi on plant use of low bioavailable soil phosphate sources was evaluated using soil microorganisms which integrated isotopic dilution techniques. Inoculation of phosphate solubilizing micro organisms increase crop yield by 510 %. Different strains of phosphatesolubilizing bacteria psb were isolated from the rhizosphere of different plants of lahore district, pakistan. In this study, isolation, screening and characterization of 35 isolates of phosphate solubilizing bacteria from different regions of karwar were carried out. Phosphatesolubilizing microorganisms associated with the. Isolation of phosphate solubilizing bacteria and fungi.
The interactive effect of phosphatesolubilizing bacteria and arbuscular mycorrhizal fungi on plant use of low bioavailable soil phosphate sources was evaluated using soil microorganisms which integrated isotopic dilution techniques. A small number of studies investigated on the occurrence of phosphate solubilizing bacteria in the marine environment and fresh water environment like lake ecosystem 3, 4. Mechanisms of phosphate solubilization by psb phosphate. Isolation of phosphate solubilizing microorganism psms from soil. Eleven soil samples were collected randomly to estimate microbial population which used plate count method. The production of organic acids by phosphate solubilizing bacteria has been well documented. Ten strains that had higher solubilization potential were selected, and they also produced indole3acetic acid, 1.
Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan b present address. Isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of oryza sativa l. Isolation and characterization of phosphate solubilizing microbes from agricultural soil t. Phosphate solubilizing biofertilizers as a component of inm many heterotropjic bacteria and fungi efficiently solubilize insoluble phosphate of soil and rock phosphates.
Isolation and identification of potential phosphate. Nagalakshmi department of microbiology, kanchi shri krishna college of arts and science, kilambi, kanchipuram631551, tn, india corresponding author abstract introduction phosphorus p is a major growthlimiting. Phosphate solubilizing activity of some bacterial strains. Phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. In this study, soil samples collected from a diverse range of slightly alkaline soil types were screened for azotobacter isolates. These bacteria may benefit crop plants like legumes, maize, and lettuce by increasing their phosphate content when inoculated alone. Immobilization of pb by phosphate solubilizing bacteria. Variations were noted in the solubilization potentials. In this study a bacterial strain was isolated from rhzhosphere soil and screened for phosphate solubilization property. Psolubilization ability of rhizosphere microorganisms is considered to be one of the most important traits associ. Phosphate solubilizing bacteria psb have the potential to enhance phosphate induced immobilization of metals to remediate contaminated soil.
The morphological, biochemical characteristics of bacterial strain was. Performance of phosphatesolubilizing bacteria in soil under. Phosphate solubilizing bacteria psb were isolated from the rhizosphere of chinese cabbage and screened on the basis of their solubilization of inorganic tricalcium phosphate in liquid cultures. Phosphate solubilizing bacteria psb are being used as biofertilizer since 1950s kudashev, 1956.
The goal of this research was to investigate the adaptation and performance of psb in conditions of high total p content in soil. Pseudomonas, bacillus, micrococcus, aspergillus, fusarium, etc. Phosphate solubilizing activities of all isolates were tested on national botanical. From our study, we conclude that the phosphate solubilizing gramnegative bacteria were present in maize, corn, jasmine, and onion fields and enrich soil soluble phosphate. It is important to explore a highefficient phosphatesolubilizing approach with a kind of microorganisms. Hence the present study on phosphatesolubilizing bacteria in the rhizosphere of foxtail millet is essentially concerned with the following aspects.
Isolation and application of salttolerant or halophilic psb will facilitate the development of salinealkali soilbased agriculture. Isolation of phosphate solubilizing bacteria psb from rhizosphere soil soil samples were prepared by inoculating 1. Potassium is a vital component of plant nutrition package limiting crop yield and. Isolation of phosphate solubilizing bacteria psb from rhizosphere soil.