Cultures were incubated at 37 c for a maximum of twelve weeks and positive cultures subcultured onto the same type of medium for identi. Traditionally, the lowenstein jensen lj medium has been used for culturing mycobacterium tuberculosis. Pdf comparison and evaluation of lowensteinjensen medium. Drug susceptibility testing dst using the lowenstein jensen lj proportional method remains the mostly used phenotypic dst method at the ugandas national tb reference laboratory with other methods such as middlebrook 7h10 mb7h10 and liquid culturebased dsts being used in research laboratories. Lowensteinjensen medium an overview sciencedirect topics. Comparison of the lowensteinjensen medium, the middlebrook. Lowensteinjensen medium for mycobacterium tuberculosis appropriate sputum. Reducing contaminated results of lowenstein jensen culture at the national tuberculosis reference laboratory nsubuga richard kasule george william medium term fellows mentors. Drugresistant tb and torres strait protected zone acquired and successfully sequenced 100 85. Refer to the document storage for more information. Jensens version eliminates congo red and uses a moderate concentration of malachite. Histopathologic examination revealed an exophytic as well as endophytic squamous cell proliferation infiltrat. Mycobacterium probe identification, broth organism is in pure culture and actively growing in broth eg, mycobacterium.
An eggmedium enriched with oligoelements, pyruvate and glutamate of sodium, and ossein is described. Lowenstein jensen lj media for the detection of mycobacterium. Jensen s version eliminates congo red and uses a moderate concentration of malachite. Thermo scientific lowensteinjensen medium lj lowenstein. This basic medium which is more sensitive than the lowensteinjensen medium, can be used for three methods of culture.
Handling and storage protective measures hands as acrobat documentves. Middlebrook 7h10 or 7h11 or albumin lowenstein jensen medium, lj. For pyrazinamide and secondline drugs, we performed drugsus. Lowenstein jensen medium is a solid medium used for the selective isolation of. Although these media support the growth of mycobacteria, several weeks 28 weeks of incubation maybe necessary before the growth can be detected. The mean duration for detecting mtb by mgit was 15. Lowenstein jensen basal medium safety data sheet date of issue 03012008. Plasmidbased highresolution melting analysis for accurate. Dec 15, 2012 the conventional culture methods such as the use of lowenstein jensen medium requires 3 to 6 weeks for its isolation, plus an additional 1 to 2 weeks for its speciation.
Hardy diagnostics lowenstein jensen media are recommended for use in the cultivation and isolation of mycobacterium species. Csir institute of microbial technology sector 39a, chandigarh 160036, india sr. Lowenstein jensen medium lowenstein jensen medium liofilchem. Staphylococcus aureus and for the detection of corynebacterium. Middlebrook 7h10 or 7h11 or lowensteinjensen medium slant or in broth eg, mycobacteria growth indicator tube 7h9 broth specimen volume. In abdominal tuberculosis tb, the reported yield from tissue culture is. All isolates resistant to the drugs on lowensteinjensen medium had mics ranging from 5 to 32 mgl for kanamycin and from 5 to 10 mgl for capreomycin. Nocardia species or other aerobic actinomycetes isolated from a primary clinical specimen, on a submitted conventional solid medium ie, lowensteinjensen. In abdominal tuberculosis tb, the reported yield from tissue culture is between 20% and 60%.
Med tech med laboratory tech knowledge assessment exam study. Wholegenome sequencing of drugresistant mycobacterium. Glycerol if required and egg mixture are added prior to the inspissation process. Dna extraction mycobacterial genomic dna was extracted and purified using qiaamp dna mini kit qiagen, usa in accordance. In this study, the diagnostic values of eggbased lowensteinjensen lj medium used extensively all over the world and ogawa 2% medium. Visible colonies are usually produced 23 weeks after incubation m. Remel lowensteinjensen gruft lj wpenicillin, nalidixic acid. Zn stain and culture on lowenstein jensen lj medium. Lowensteinjensen middlebrook 7h9 broth en middlebrook 7h10 agar en. Visible growth of isolate on solid media isolate in broth media.
Loeffler medium it is an inspissated medium used for the demonstration of chromogenesis example. Growth media number growth name 1 1 castenholz tye medium 2 2 r2ye 3 3 nutrient agar 4 5 yepd 5 6 malt yeast agar 6 7 potato dextrose agar 7 8 yeast glucose agar 8 9 yeast mannitol agar 9 11 mrs medium 10 12 tomato juice agar 11 postgates medium. Nocardia, aerobic actinomycetes susceptibility ast. Jensen 8 modified lowensteins medium by altering the citrate and phosphate contents, eliminating the congo red dye and by increasing the malachite green concentration. Medium with the addition of two antimicrobics to increase. The foremost benefit of cbnaat for diagnosis of osteoarticular tuberculosis was that. Lowensteinjensen medium base is a relatively simple formulation that requires supplementation in order to support the growth of mycobacteria. Other solid media techniques 7 h9, 7 h10, and 7 h11 have lower contaminations rates but are, in general, too. Tuberculosis in tanzanian wildlife university of minnesota. This duration may further be prolonged in the case of paucibacillary specimens. Such a prolonged turnaround time in the diagnosis is unacceptable, as rapid detection and identification of mtb is essential both for medical and epidemiological purposes 7. To evaluate the performance of lowensteinjensen medium lj supplemented with pyruvate and glycerol ljpg. Positive tsal medium culture was adopted as the final.
For rapidly growing mycobacteria rgm, the panel of drugs included clarithromycin, amika. Pulmonary tuberculosis detailed history of presenting illness hpi chronic productive cough occasional haemoptysis haemoptysis red flag resistant to amoxicillin if previously treated for bronchitis with antibiotics recent onset of night sweats. Determination criteria for positivenegative grading criteria for microscopy of mtbl, please refer to the study per formed by skrahin et al. Medium was originally formulated by lowenstein, containing congo red and malachite green dyes 10. The second part was stored at 200c in deep freezer until processed for pcr within 24 hours. Lowenstein jensen with iron is used to determine iron uptake as a means of differentiation between slow and rapid growing mycobacterium species. Pdf performance comparison of a pair of lowensteinjensen. Different culture media and their uses flashcards quizlet. Of the 19 samples that were positive for mtb using cbnaat, 3 15. Two isolates that were kanamycin susceptible on lowensteinjensen medium were found to be resistant using bactec mgit 960 mic 5 mgl. The conventional culture methods such as the use of lowenstein jensen medium requires 3 to 6 weeks for its isolation, plus an additional 1 to 2 weeks for its speciation. The samples were processed as per standard microbiological procedure.
Lowenstein jensen with pyruvate incorporates pyruvic acid into the lj basal medium to stimulate the growth of m. Medical technicianmedical laboratory technologist mtmlt. Bbl lowensteinjensen medium bbl lowensteinjensen medium with 5% sodium chloride. Comparative study of smear microscopy, rapid slide culture.
Traditional solid culture media lowensteinjensen or ogawa are more sensitive than afb microscopy, but require from 23 weeks to several months to yield colonies of m. This culture medium complies with recommendations of the bull. Professionals lawyers access the profiles of the more than 350 lowenstein sandler lawyers who provide critical legal counsel to clients in virtually every sector of the global economy. Keep well closed and protected from direct sunlight and moisture. Thermo scientific remel lowensteinjensen medium with 2. Lowensteinjensen selective medium for reducing contamination. Escherichia coli, slamonella and shigella are inhibited in the medium while klebsiella, citrobacter and proteus grow freely. Lowenstein jensen lj medium, and following the clinical and laboratory standards ins titutes document m24a2 20 and manufacturers instructions for drug susceptibility testing sigmachemical co. Original article study on drug resistance and mycobacterium. Lowenstein jensen medium base is a relatively simple formulation that requires supplementation in order to support the growth of mycobacteria. Original article pathogenicity and susceptibility profile of. The generation time of tb is approximately 1218 hours, so that cultures must be incubated for three to six weeks at 370c until proliferation becomes microscopically visible. Nocardia species or other aerobic actinomycetes isolated from a primary clinical specimen, on a submitted conventional solid medium ie, lowenstein jensen lj slant, sabouraud dextrose sab slant, or similar. Musisi kenneth mohntrl dr eric ikoona ministry of health medium term fellowship dissemination workshop speke resort, munyonyo.
Part of tissue sample was sent to pathology laboratory for relevant cytopathological and histopathological investigations. Pdf comparison of mycobacterium tuberculosis culture using. The original formulation of lowenstein jensen media was developed by lowenstein who incorporated congo red and malachite green to inhibit unwanted bacteria. Crossborder movement of highly drugresistant mycobacterium.